1w9h | pdb_00001w9h
THE STRUCTURE OF A PIWI PROTEIN FROM ARCHAEOGLOBUS FULGIDUS.
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Overview
RNA silencing regulates gene expression through mRNA degradation, translation repression and chromatin remodelling. The fundamental engines, of RNA silencing are RISC and RITS complexes, whose common components are, 21-25 nt RNA and an Argonaute protein containing a PIWI domain of unknown, function. The crystal structure of an archaeal Piwi protein (AfPiwi) is, organised into two domains, one resembling the sugar-binding portion of, the lac repressor and another with similarity to RNase H. Invariant, residues and a coordinated metal ion lie in a pocket that surrounds the, conserved C-terminus of the protein, defining a key functional region in, the PIWI domain. Furthermore, two Asp residues, conserved in the majority, of Argonaute sequences, align spatially with the catalytic Asp residues ... [(full description)]
About this Structure
1W9H is a [Single protein] structure of sequence from [Archaeoglobus fulgidus] with CD, NI and CL as [ligands]. Full crystallographic information is available from [OCA].
Reference
Crystal structure of a PIWI protein suggests mechanisms for siRNA recognition and slicer activity., Parker JS, Roe SM, Barford D, EMBO J. 2004 Dec 8;23(24):4727-37. Epub 2004 Nov 25. PMID:15565169
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