1z5u | pdb_00001z5u
Crystal structure of S. typhimurium AphA complexed with cyclic-AMP
|
Overview
The aphA gene of Salmonella enterica sv. Typhimurium strain MD6001 was, cloned in the multicopy plasmid pBluescript SK(-). The recombinant AphA, protein was purified to homogeneity. The protein crystallized in the, orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a =, 112.4, b = 130.2, c = 139.6 A. Consistent with the self-rotation function, there are two tetramers in the asymmetric unit, indicating a solvent, content of approximately 54%. The crystals are composed of biologically, active AphA molecules.
About this Structure
1Z5U is a Single protein structure of sequence from Salmonella typhimurium with MG and CMP as ligands. Active as Acid phosphatase, with EC number 3.1.3.2 Full crystallographic information is available from OCA.
Reference
Expression, purification, crystallization and preliminary X-ray diffraction studies of recombinant class B non-specific acid phosphatase of Salmonella typhimurium., Makde RD, Kumar V, Gupta GD, Jasti J, Singh TP, Mahajan SK, Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1849-52. Epub 2003, Sep 19. PMID:14501135
Page seeded by OCA on Wed Nov 21 07:15:25 2007
