2bk0 | pdb_00002bk0
CRYSTAL STRUCTURE OF THE MAJOR CELERY ALLERGEN API G 1
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Overview
Many patients who have been sensitised to pollen, display allergic, symptoms after ingestion of certain plant food such as fresh fruit, vegetables and nuts. The cause is the cross-reactivity between, structurally very similar major plant allergens. In particular, allergy to, celery is very frequently associated with birch and mugwort pollen, sensitization, known as to the birch-mugwort-celery syndrome. The crystal, structure of the major celery allergen Api g 1, a homologue of the major, birch pollen allergen Bet v 1, has been determined to a resolution of 2.9, A. The structure of Api g 1 is very similar to that of Bet v 1 with major, differences occurring in the segment comprised of residues 23-45, preceding the well conserved glycine-rich P-loop, as well as in loops, beta3-beta4 and beta5-beta6. In particular, Api g 1 lacks E45, which has, been shown to be a crucial residue for antibody recognition in the crystal, complex of Bet v 1 with the Fab fragment of a murine monoclonal IgG (BV16), antibody. The absence of E45 and the structural differences in the, preceding segment suggest that this region of the Api g 1 surface is, probably not responsible for the observed cross-reactivity with Bet v 1. A, detailed analysis of the molecular surface in combination with sequence, alignment revealed three conserved surface patches which may account for, cross-reactivity with Bet v 1. Several residues of Bet v 1 which have been, shown by mutagenesis studies to be involved in IgE recognition belong to, these conserved surface regions. The structure of Api g 1 and the related, epitope analysis provides a molecular basis for a better understanding of, allergen cross-reactivity and may lead to the development of hypoallergens, which would allow a safer immunotherapy.
About this Structure
2BK0 is a Single protein structure of sequence from Apium graveolens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the major celery allergen Api g 1: molecular analysis of cross-reactivity., Schirmer T, Hoffimann-Sommergrube K, Susani M, Breiteneder H, Markovic-Housley Z, J Mol Biol. 2005 Sep 2;351(5):1101-9. PMID:16051263
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