2fd4 | pdb_00002fd4
Crystal Structure of AvrPtoB (436-553)
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Overview
The Pseudomonas syringae protein AvrPtoB is translocated into plant cells, where it inhibits immunity-associated programmed cell death (PCD). The, structure of a C-terminal domain of AvrPtoB that is essential for anti-PCD, activity reveals an unexpected homology to the U-box and RING-finger, components of eukaryotic E3 ubiquitin ligases, and we show that AvrPtoB, has ubiquitin ligase activity. Mutation of conserved residues involved in, the binding of E2 ubiquitin-conjugating enzymes abolishes this activity in, vitro, as well as anti-PCD activity in tomato leaves, which dramatically, decreases virulence. These results show that Pseudomonas syringae uses a, mimic of host E3 ubiquitin ligases to inactivate plant defenses.
About this Structure
2FD4 is a Single protein structure of sequence from Pseudomonas syringae. Full crystallographic information is available from OCA.
Reference
A bacterial inhibitor of host programmed cell death defenses is an E3 ubiquitin ligase., Janjusevic R, Abramovitch RB, Martin GB, Stebbins CE, Science. 2006 Jan 13;311(5758):222-6. Epub 2005 Dec 22. PMID:16373536
Page seeded by OCA on Wed Nov 21 10:29:20 2007
