2gjw | pdb_00002gjw
RNA Recognition and Cleavage by an Splicing Endonuclease
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Overview
The RNA splicing endonuclease cleaves two phosphodiester bonds within, folded precursor RNAs during intron removal, producing the functional RNAs, required for protein synthesis. Here we describe at a resolution of 2.85, angstroms the structure of a splicing endonuclease from Archaeglobus, fulgidus bound with a bulge-helix-bulge RNA containing a noncleaved and a, cleaved splice site. The endonuclease dimer cooperatively recognized a, flipped-out bulge base and stabilizes sharply bent bulge backbones that, are poised for an in-line RNA cleavage reaction. Cooperativity arises, because an arginine pair from one catalytic domain sandwiches a nucleobase, within the bulge cleaved by the other catalytic domain.
About this Structure
2GJW is a Single protein structure of sequence from Archaeoglobus fulgidus. Active as tRNA-intron endonuclease, with EC number 3.1.27.9 Full crystallographic information is available from OCA.
Reference
RNA recognition and cleavage by a splicing endonuclease., Xue S, Calvin K, Li H, Science. 2006 May 12;312(5775):906-10. PMID:16690865
Page seeded by OCA on Wed Nov 21 11:14:47 2007
