Sandbox313
Lipase (1lpm)
Mark Omobono, 03.02.09
Lipase is a single-stranded enzyme that is responsible for breaking down the majority of the dietary lipids (triglycerides, fats, oils) digested by most living organisms. Lipases are located in the digestive juices and general digestive regions of an organism. They are also used in industry as yogurt and cheese fermentation vehicles, as well as more modern applications such as converting vegetable oil to usable fuel.
Lipases catalyze the hydrolysis of ester bonds in lipids by employing a chymotrypsin-like hydrolysis mechanism. The enzyme itself is made up of both alpha and beta sheets, but the enzyme activity hinges upon an "alpha-beta hydrolase fold". Characteristics of this fold lie in a catalytic triad, all the pieces of which are located on loops.These include the reactive phosphonate center, two calcium ions, and two sugars on the outside of the folded molecule.
The molecule retains its hydrophobic aspects when folded, making this a well-strucured enzyme.
Full crystallographic information is available from OCA.
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| 1lpm, resolution 2.18Å (default scene) | |||||||||||||
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| Ligands: | CA, MPA, NAG | ||||||||||||
| Activity: | Triacylglycerol lipase, with EC number 3.1.1.3 | ||||||||||||
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| Resources: | FirstGlance, OCA, RCSB, PDBsum | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
