2nv6 | pdb_00002nv6
Mycobacterium tuberculosis InhA (S94A) bound with INH-NAD adduct
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Overview
Isoniazid is one of the most effective antituberculosis drugs, yet its, precise mechanism of action is still controversial. Using specialized, linkage transduction, a single point mutation allele (S94A) within the, putative target gene inhA was transferred in Mycobacterium tuberculosis., The inhA(S94A) allele was sufficient to confer clinically relevant levels, of resistance to isoniazid killing and inhibition of mycolic acid, biosynthesis. This resistance correlated with the decreased binding of the, INH-NAD inhibitor to InhA, as shown by enzymatic and X-ray, crystallographic analyses, and establishes InhA as the primary target of, isoniazid action in M. tuberculosis.
About this Structure
2NV6 is a Single protein structure of sequence from Mycobacterium tuberculosis with ZID as ligand. Active as [acyl-carrier-protein_reductase_(NADH) Enoyl-[acyl-carrier-protein] reductase (NADH)], with EC number 1.3.1.9 Full crystallographic information is available from OCA.
Reference
Transfer of a point mutation in Mycobacterium tuberculosis inhA resolves the target of isoniazid., Vilcheze C, Wang F, Arai M, Hazbon MH, Colangeli R, Kremer L, Weisbrod TR, Alland D, Sacchettini JC, Jacobs WR Jr, Nat Med. 2006 Sep;12(9):1027-9. Epub 2006 Aug 13. PMID:16906155 [[Category: Enoyl-[acyl-carrier-protein] reductase (NADH)]]
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