1r3g | pdb_00001r3g

1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues

File:1r3g.gif

spinqualitylabelsall models 1r3g, resolution 1.16Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D

Overview

[structure: see text] Oligonucleotides with a novel, 2'-O-[2-(guanidinium)ethyl] (2'-O-GE) modification have been synthesized, using a novel protecting group strategy for the guanidinium group. This, modification enhances the binding affinity of oligonucleotides to RNA as, well as duplex DNA (DeltaT(m) 3.2 degrees C per modification). The 2'-O-GE, modified oligonucleotides exhibited exceptional resistance to nuclease, degradation. The crystal structure of a palindromic duplex formed by a DNA, oligonucleotide with a single 2'-O-GE modification was solved at 1.16 A, resolution.

About this Structure

1R3G is a Protein complex structure of sequences from [1] with MG as ligand. Full crystallographic information is available from OCA.

Reference

2'-O-[2-(guanidinium)ethyl]-modified oligonucleotides: stabilizing effect on duplex and triplex structures., Prakash TP, Puschl A, Lesnik E, Mohan V, Tereshko V, Egli M, Manoharan M, Org Lett. 2004 Jun 10;6(12):1971-4. PMID:15176796

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