1s2r | pdb_00001s2r
A High Resolution Crystal Structure of [d(CGCAAATTTGCG)]2
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Overview
The crystal structure of [d(CGCAAATTTGCG)]2 has been determined to 1.5 Å resolution, representing the first high-resolution structure of this DNA fragment. The ion interactions are novel. A spermine molecule replaces a Mg2+ observed in analogous structures. Unlike lower-resolution structures, the minor groove is narrow and the major groove lacks extra Watson-Crick hydrogen bonds. In addition, a monolayer of solvent sites, including a "spine of hydration", is visible in the minor groove. The crystal of [d(CGCAAATTTGCG)]2 was grown from a solution containing spermine, magnesium, and lithium. The conformation recapitulates that of "monovalent-minus" DNA.
About this Structure
1S2R is a Protein complex structure of sequences from [1] with SPM as ligand. Full crystallographic information is available from OCA.
Reference
High-resolution structure of an extended A-tract: [d(CGCAAATTTGCG)]2., Woods KK, Maehigashi T, Howerton SB, Sines CC, Tannenbaum S, Williams LD, J Am Chem Soc. 2004 Dec 1;126(47):15330-1. PMID:15563130
Page seeded by OCA on Sat Nov 24 23:47:29 2007
