Structural highlights
Publication Abstract from PubMed
The integration of the human immunodeficiency virus type 1 DNA into the host cell genome is catalysed by the viral integrase (IN). The reaction consists of a 3'-processing [dinucleotide released from each 3' end of the viral long terminal repeat (LTR)] followed by a strand transfer (insertion of the viral genome into the human chromosome). A 17 base pair oligonucleotide d(GGAAAATCTCTAGCAGT), d(ACTGCTAGAGATTTTCC) reproducing the U5-LTR extremity of viral DNA that contains the IN attachment site was analysed by NMR using the classical NOEs and scalar coupling constants in conjunction with a small set of residual dipolar coupling constants (RDCs) measured at the 13C/15N natural abundance. The combination of these two types of parameters in calculations significantly improved the DNA structure determination. The well-known features of A-tracts were clearly identified by RDCs in the first part of the molecule. The binding/cleavage site at the viral DNA end is distinguishable by a loss of regular base stacking and a distorted minor groove that can aid its specific recognition by IN.
Pre-organized structure of viral DNA at the binding-processing site of HIV-1 integrase.,Renisio JG, Cosquer S, Cherrak I, El Antri S, Mauffret O, Fermandjian S Nucleic Acids Res. 2005 Apr 6;33(6):1970-81. Print 2005. PMID:15814814[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Renisio JG, Cosquer S, Cherrak I, El Antri S, Mauffret O, Fermandjian S. Pre-organized structure of viral DNA at the binding-processing site of HIV-1 integrase. Nucleic Acids Res. 2005 Apr 6;33(6):1970-81. Print 2005. PMID:15814814 doi:33/6/1970
