| Structural highlights
Function
CRA_ECOLI Global transcriptional regulator, which plays an important role in the regulation of carbon metabolism. Activates transcription of genes encoding biosynthetic and oxidative enzymes (involved in Krebs cycle, glyoxylate shunt and gluconeogenesis, such as ppsA and fbp). Represses genes involved in sugar catabolism, such as fruB, pfkA, pykF and adhE. Binds asymmetrically to the two half-sites of its operator.[1] [2] [3] [4] [5] [6] [7] [8]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
- ↑ Shimada T, Fujita N, Maeda M, Ishihama A. Systematic search for the Cra-binding promoters using genomic SELEX system. Genes Cells. 2005 Sep;10(9):907-18. PMID:16115199 doi:http://dx.doi.org/10.1111/j.1365-2443.2005.00888.x
- ↑ Cortay JC, Negre D, Scarabel M, Ramseier TM, Vartak NB, Reizer J, Saier MH Jr, Cozzone AJ. In vitro asymmetric binding of the pleiotropic regulatory protein, FruR, to the ace operator controlling glyoxylate shunt enzyme synthesis. J Biol Chem. 1994 May 27;269(21):14885-91. PMID:8195118
- ↑ Ramseier TM, Negre D, Cortay JC, Scarabel M, Cozzone AJ, Saier MH Jr. In vitro binding of the pleiotropic transcriptional regulatory protein, FruR, to the fru, pps, ace, pts and icd operons of Escherichia coli and Salmonella typhimurium. J Mol Biol. 1993 Nov 5;234(1):28-44. PMID:8230205 doi:http://dx.doi.org/10.1006/jmbi.1993.1561
- ↑ Bledig SA, Ramseier TM, Saier MH Jr. Frur mediates catabolite activation of pyruvate kinase (pykF) gene expression in Escherichia coli. J Bacteriol. 1996 Jan;178(1):280-3. PMID:8550429
- ↑ Ramseier TM, Bledig S, Michotey V, Feghali R, Saier MH Jr. The global regulatory protein FruR modulates the direction of carbon flow in Escherichia coli. Mol Microbiol. 1995 Jun;16(6):1157-69. PMID:8577250
- ↑ Negre D, Bonod-Bidaud C, Geourjon C, Deleage G, Cozzone AJ, Cortay JC. Definition of a consensus DNA-binding site for the Escherichia coli pleiotropic regulatory protein, FruR. Mol Microbiol. 1996 Jul;21(2):257-66. PMID:8858581
- ↑ Ramseier TM. Cra and the control of carbon flux via metabolic pathways. Res Microbiol. 1996 Jul-Sep;147(6-7):489-93. PMID:9084760
- ↑ Mikulskis A, Aristarkhov A, Lin EC. Regulation of expression of the ethanol dehydrogenase gene (adhE) in Escherichia coli by catabolite repressor activator protein Cra. J Bacteriol. 1997 Nov;179(22):7129-34. PMID:9371462
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