25hs

Cryo-EM structure of Pseudomonas aeruginosa FtsQBLWI in complex with aztreonam

spinqualitylabelsall models 25hs, resolution 3.12Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 25hs is a 5 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.12Å Ligands: AZR Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function FTSL_PSEAE Essential cell division protein. May link together the upstream cell division proteins, which are predominantly cytoplasmic, with the downstream cell division proteins, which are predominantly periplasmic. Publication Abstract from PubMed Septal peptidoglycan (sPG) biosynthesis during bacterial cell division is driven by the dynamic divisome complex. Its core components, glycosyltransferase FtsW and transpeptidase FtsI are responsible for glycan chain polymerization and crosslinking, respectively. FtsI is also the target of beta-lactams. The essential membrane complex FtsQ-FtsB-FtsL regulates FtsWI enzymatic activity. However, the mechanism of FtsQBLWI-mediated sPG synthesis and beta-lactam-induced conformational changes have remained elusive. Here, we present cryo-electron microscopy (cryo-EM) structures of the Pseudomonas aeruginosa FtsQBLWI complex in the apo state and bound to aztreonam or imipenem. Our work reveals intricate structural details, including the putative substrate-binding cavities of FtsW, FtsI-mediated allosteric activation of FtsW, and beta-lactam-triggered conformational rearrangements. Collectively, these structural, genetic and biochemical analyses reveal the mechanism of FtsQBLWI-controlled sPG synthesis and beta-lactam action on this complex, providing a molecular basis for optimizing existing beta-lactams and developing novel antibiotics. Structural, functional, and mechanistic studies of the bacterial divisome FtsWIQBL in complex with antibiotics.,Zhu S, Hu Y, Wang R, Li D, Zhang Z, Dong C Structure. 2026 Jul 31:S0969-2126(26)00215-7. doi: 10.1016/j.str.2026.07.005. PMID:42537644 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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