2mhh

Solution structure of a EF-hand domain from sea urchin polycystin-2

spinqualitylabelsall models 2mhh Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 2mhh is a 1 chain structure with sequence from Strongylocentrotus purpuratus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q7Z2B5_STRPU Publication Abstract from PubMed Polycystin 2 (PC2) is a calcium-dependent calcium channel, and mutations to human PC2 (hPC2) are associated with polycystic kidney disease. The C-terminal tail of hPC2 contains 2 EF hand motifs, but only the second binds calcium. Here, we investigate whether these EF hand motifs serve as a calcium sensor responsible for the calcium dependence of PC2 function. Using NMR and bioinformatics, we show that the overall fold is highly conserved, but in evolutionarily earlier species, both EF hands bind calcium. To test whether the EF hand motif is truly a calcium sensor controlling PC2 channel function, we altered the number of calcium binding sites in hPC2. NMR studies confirmed that modified hPC2 binds an additional calcium ion. Single-channel recordings demonstrated a leftward shift in the calcium dependence, and imaging studies in cells showed that calcium transients were enhanced compared with wild-type hPC2. However, biophysics and functional studies showed that the first EF hand can only bind calcium and be functionally active if the second (native) calcium-binding EF hand is intact. These results suggest that the number and location of calcium-binding sites in the EF hand senses the concentration of calcium required for PC2 channel activity and cellular function. The number and location of EF hand motifs dictates the calcium dependence of polycystin-2 function.,Kuo IY, Keeler C, Corbin R, Celic A, Petri ET, Hodsdon ME, Ehrlich BE FASEB J. 2014 May;28(5):2332-46. doi: 10.1096/fj.13-247106. Epub 2014 Feb 20. PMID:24558196 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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