3b2m

Crystal Structure of the Major Pilin from Streptococcus pyogenes

spinqualitylabelsall models 3b2m, resolution 2.22Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3b2m is a 3 chain structure with sequence from Streptococcus pyogenes serotype M1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.22Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PILIN_STRP1 Major component of the pilus. A stack of the pilin subunits, joined by intermolecular isopeptide bonds, forms the pilus. The pilus is required for bacterial adhesion to host cells, for bacterial aggregation, and for biofilm formation. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Many bacterial pathogens have long, slender pili through which they adhere to host cells. The crystal structure of the major pilin subunit from the Gram-positive human pathogen Streptococcus pyogenes at 2.2 angstroms resolution reveals an extended structure comprising two all-beta domains. The molecules associate in columns through the crystal, with each carboxyl terminus adjacent to a conserved lysine of the next molecule. This lysine forms the isopeptide bonds that link the subunits in native pili, validating the relevance of the crystal assembly. Each subunit contains two lysine-asparagine isopeptide bonds generated by an intramolecular reaction, and we find evidence for similar isopeptide bonds in other cell surface proteins of Gram-positive bacteria. The present structure explains the strength and stability of such Gram-positive pili and could facilitate vaccine development. Stabilizing isopeptide bonds revealed in gram-positive bacterial pilus structure.,Kang HJ, Coulibaly F, Clow F, Proft T, Baker EN Science. 2007 Dec 7;318(5856):1625-8. PMID:18063798 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Pilin 3D structures

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