3eyy

Structural basis for the specialization of Nur, a nickel-specific Fur homologue, in metal sensing and DNA recognition

spinqualitylabelsall models 3eyy, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3eyy is a 2 chain structure with sequence from Streptomyces coelicolor. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.4Å Ligands: CL, EDO, MLI, NI, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q9K4F8_STRCO Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Nur, a member of the Fur family, is a nickel-responsive transcription factor that controls nickel homeostasis and anti-oxidative response in Streptomyces coelicolor. Here we report the 2.4-A resolution crystal structure of Nur. It contains a unique nickel-specific metal site in addition to a nonspecific common metal site. The identification of the 6-5-6 motif of the Nur recognition box and a Nur/DNA complex model reveals that Nur mainly interacts with terminal bases of the palindrome on complex formation. This contrasts with more distributed contacts between Fur and the n-1-n type of the Fur-binding motif. The disparity between Nur and Fur in the conformation of the S1-S2 sheet in the DNA-binding domain can explain their different DNA-recognition patterns. Furthermore, the fact that the specificity of Nur in metal sensing and DNA recognition is conferred by the specific metal site suggests that its introduction drives the evolution of Nur orthologs in the Fur family. Structural basis for the specialization of Nur, a nickel-specific Fur homolog, in metal sensing and DNA recognition.,An YJ, Ahn BE, Han AR, Kim HM, Chung KM, Shin JH, Cho YB, Roe JH, Cha SS Nucleic Acids Res. 2009 Jun;37(10):3442-51. Epub 2009 Mar 31. PMID:19336416 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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