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3lnr

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Crystal structure of poly-HAMP domains from the P. aeruginosa soluble receptor Aer2

Structural highlights

3lnr is a 1 chain structure with sequence from Pseudomonas aeruginosa PAO1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.64Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MCPB_PSEAE Chemoreceptor that plays a critical role in the virulence and pathogenesis of P.aeruginosa in a variety of hosts (PubMed:31511598). Probably acts through oxygen sensing (PubMed:31511598, PubMed:28167524, PubMed:34383467). Uses a heme-based sensor (PubMed:21255112, PubMed:22622145). Could be involved in chemotaxis (PubMed:12142407, PubMed:14987771). When expressed in E.coli, is able to sense and mediate repellent responses to oxygen, carbon monoxide and nitric oxide (PubMed:21255112).^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

↑ Ferrandez A, Hawkins AC, Summerfield DT, Harwood CS. Cluster II che genes from Pseudomonas aeruginosa are required for an optimal chemotactic response. J Bacteriol. 2002 Aug;184(16):4374-83. PMID:12142407
↑ Hong CS, Shitashiro M, Kuroda A, Ikeda T, Takiguchi N, Ohtake H, Kato J. Chemotaxis proteins and transducers for aerotaxis in Pseudomonas aeruginosa. FEMS Microbiol Lett. 2004 Feb 16;231(2):247-52. doi:, 10.1016/S0378-1097(04)00009-6. PMID:14987771 doi:http://dx.doi.org/10.1016/S0378-1097(04)00009-6
↑ Watts KJ, Taylor BL, Johnson MS. PAS/poly-HAMP signalling in Aer-2, a soluble haem-based sensor. Mol Microbiol. 2011 Feb;79(3):686-99. doi: 10.1111/j.1365-2958.2010.07477.x. Epub, 2010 Dec 7. PMID:21255112 doi:http://dx.doi.org/10.1111/j.1365-2958.2010.07477.x
↑ Sawai H, Sugimoto H, Shiro Y, Ishikawa H, Mizutani Y, Aono S. Structural basis for oxygen sensing and signal transduction of the heme-based sensor protein Aer2 from Pseudomonas aeruginosa. Chem Commun (Camb). 2012 Jul 4;48(52):6523-5. doi: 10.1039/c2cc32549g. Epub 2012 , May 23. PMID:22622145 doi:10.1039/c2cc32549g
↑ Garcia D, Orillard E, Johnson MS, Watts KJ. Gas Sensing and Signaling in the PAS-Heme Domain of the Pseudomonas aeruginosa Aer2 Receptor. J Bacteriol. 2017 Aug 22;199(18). pii: JB.00003-17. doi: 10.1128/JB.00003-17., Print 2017 Sep 15. PMID:28167524 doi:http://dx.doi.org/10.1128/JB.00003-17
↑ Garcia-Fontana C, Vilchez JI, Gonzalez-Requena M, Gonzalez-Lopez J, Krell T, Matilla MA, Manzanera M. The involvement of McpB chemoreceptor from Pseudomonas aeruginosa PAO1 in virulence. Sci Rep. 2019 Sep 11;9(1):13166. doi: 10.1038/s41598-019-49697-7. PMID:31511598 doi:http://dx.doi.org/10.1038/s41598-019-49697-7
↑ Orillard E, Anaya S, Johnson MS, Watts KJ. Oxygen-Induced Conformational Changes in the PAS-Heme Domain of the Pseudomonas aeruginosa Aer2 Receptor. Biochemistry. 2021 Aug 31;60(34):2610-2622. doi: 10.1021/acs.biochem.1c00452., Epub 2021 Aug 12. PMID:34383467 doi:http://dx.doi.org/10.1021/acs.biochem.1c00452