3wid

Structure of a glucose dehydrogenase T277F mutant in complex with NADP

spinqualitylabelsall models 3wid, resolution 2.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3wid is a 4 chain structure with sequence from Thermoplasma volcanium GSS1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.25Å Ligands: 1PE, NAP, PGO, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q979W2_THEVO Catalyzes the NAD(P)(+)-dependent oxidation of D-glucose to D-gluconate via gluconolactone. Can utilize both NAD(+) and NADP(+) as electron acceptor. Is involved in the degradation of glucose through a non-phosphorylative variant of the Entner-Doudoroff pathway (By similarity).[HAMAP-Rule:MF_02127] Publication Abstract from PubMed Glucose dehydrogenase from the thermoacidophilic archaeon Thermoplasma volcanium (tvGlcDH) is highly active towards D-glucose and D-galactose, but does not utilize aldopentoses such as D-xylose as substrates. In the present study, the crystal structures of substrate/cofactor-free tvGlcDH and of a tvGlcDH T277F mutant in a binary complex with NADP and in a ternary complex with D-glucose and nicotinic acid adenine dinucleotide phosphate, an NADP analogue, were determined at resolutions of 2.6, 2.25 and 2.33 A, respectively. The overall structure of each monomer showed notable similarity to that of the enzyme from Sulfolobus solfataricus (ssGlcDH-1), which accepts a broad range of C5 and C6 sugars as substrates. However, the amino-acid residues of tvGlcDH involved in substrate binding markedly differed from those of ssGlcDH-1. Structural comparison revealed that a decreased number of interactions between the C3-hydroxyl group of the sugar and the enzyme are likely to be responsible for the lack of reactivity of tvGlcDH towards D-xylose. Structural insight into glucose dehydrogenase from the thermoacidophilic archaeon Thermoplasma volcanium.,Kanoh Y, Uehara S, Iwata H, Yoneda K, Ohshima T, Sakuraba H Acta Crystallogr D Biol Crystallogr. 2014 May;70(Pt 5):1271-80. doi:, 10.1107/S1399004714002363. Epub 2014 Apr 29. PMID:24816096 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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