4h18

Three dimensional structure of corynomycoloyl tranferase C

spinqualitylabelsall models 4h18, resolution 1.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4h18 is a 4 chain structure with sequence from Corynebacterium glutamicum ATCC 13032. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.755Å Ligands: MG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8NTG4_CORGL Publication Abstract from PubMed We have previously described the posttranslational modification of pore-forming small proteins of Corynebacterium by mycolic acid, a very-long-chain alpha-alkyl and beta-hydroxy fatty acid. Using a combination of chemical analyses and mass spectrometry, we identified the mycoloyl transferase (Myt) that catalyzes the transfer of the fatty acid residue to yield O-acylated polypeptides. Inactivation of corynomycoloyl transferase C (cg0413 [Corynebacterium glutamicum mytC {CgmytC}]), one of the six Cgmyt genes of C. glutamicum, specifically abolished the O-modification of the pore-forming proteins PorA and PorH, which is critical for their biological activity. Expectedly, complementation of the cg0413 mutant with either the wild-type gene or its orthologues from Corynebacterium diphtheriae and Rhodococcus, but not Nocardia, fully restored the O-acylation of the porins. Consistently, the three-dimensional structure of CgMytC showed the presence of a unique loop that is absent from enzymes that transfer mycoloyl residues onto both trehalose and the cell wall arabinogalactan. These data suggest the implication of this structure in the enzyme specificity for protein instead of carbohydrate. Identification of a mycoloyl transferase selectively involved in o-acylation of polypeptides in corynebacteriales.,Huc E, de Sousa-D'Auria C, de la Sierra-Gallay IL, Salmeron C, van Tilbeurgh H, Bayan N, Houssin C, Daffe M, Tropis M J Bacteriol. 2013 Sep;195(18):4121-8. doi: 10.1128/JB.00285-13. Epub 2013 Jul 12. PMID:23852866 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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