4irm

Crystal structure of mntc r116a mutant exhibits flexibility in the c-terminal domain

spinqualitylabelsall models 4irm, resolution 3.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4irm is a 3 chain structure with sequence from Synechocystis sp. PCC 6803. This structure supersedes the now removed PDB entry 3v63. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 3.5Å Ligands: MN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q79EF9_SYNY3 Publication Abstract from PubMed The cyanobacterium Synechocystis sp. PCC 6803 imports Mn2+ ions via MntCAB, an ABC transport system that is expressed at submicromolar Mn2+ concentrations. The structures of the wild type (WT) and a site-directed mutant of the MntC solute-binding protein have been determined at 2.7 and 3.5 A resolution, respectively. The WT structure is significantly improved over the previously determined structure (PDB entry 1xvl), showing improved Mn2+ binding site parameters, disulfide bonds in all three monomers and ions bound to the protein surface, revealing the role of Zn2+ ions in the crystallization liquor. The structure of MntC reveals that the active site is surrounded by neutral-to-positive electrostatic potential and is dominated by a network of polar interactions centred around Arg116. The mutation of this residue to alanine was shown to destabilize loops in the entrance to the metal-ion binding site and suggests a possible role in MntC function. Arginine 116 stabilizes the entrance to the metal ion-binding site of the MntC protein.,Kanteev M, Adir N Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Mar 1;69(Pt 3):237-42., doi: 10.1107/S174430911300153X. Epub 2013 Feb 22. PMID:23519795 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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