4twl

Crystal structure of dioscorin complexed with ascorbate

Structural highlights

4twl is a 2 chain structure with sequence from Dioscorea japonica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.11Å
Ligands:	ASC, SO4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DIOA3_DIOJA Storage protein of tuber. Involved in protection against oxidative stress (Probable). Has carbonate dehydratase, trypsin inhibitor, dehydroascorbate (DHA) reductase and monodehydroascorbate (MDA) reductase activities (PubMed:22195572). Catalyzes the reactions of carbonate dehydratase and DHA reductase independently of zinc and glutathione (GSH). The coupled reaction is capable of recycling a plant antioxidant ascorbate using ubiquitous compounds H(2)O and CO(2) (PubMed:25747844). Exhibits antioxidant activity. Able to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. Exhibits immunomodulatory activity. Activates Toll-like receptor 4 signaling pathways by up-regulating the gene expression of pro-inflammatory cytokines, such as tumor necrosis factor alpha, interleukin-1 beta and interleukin-6, and chemokines RANTES and MCP-1, in mouse RAW 264.7 macrophages. Stimulates the phagocytosis of E.coli by the LPS-treated mouse macrophages (PubMed:22796748).^[1] ^[2] ^[3]

References

↑ Xue YL, Miyakawa T, Sawano Y, Tanokura M. Cloning of genes and enzymatic characterizations of novel dioscorin isoforms from Dioscorea japonica. Plant Sci. 2012 Feb;183:14-9. PMID:22195572 doi:10.1016/j.plantsci.2011.10.021
↑ Jheng YJ, Tsai WY, Chen KH, Lin KW, Chyan CL, Yang CC, Lin KC. Recombinant dioscorins of the yam storage protein expressed in Escherichia coli exhibit antioxidant and immunomodulatory activities. Protein Expr Purif. 2012 Sep;85(1):77-85. PMID:22796748 doi:10.1016/j.pep.2012.07.001
↑ Xue YL, Miyakawa T, Nakamura A, Hatano K, Sawano Y, Tanokura M. Yam Tuber Storage Protein Reduces Plant Oxidants Using the Coupled Reactions as Carbonic Anhydrase and Dehydroascorbate Reductase. Mol Plant. 2015 Jul;8(7):1115-8. PMID:25747844 doi:10.1016/j.molp.2015.02.015

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OCA

[1] Xue YL, Miyakawa T, Sawano Y, Tanokura M. Cloning of genes and enzymatic characterizations of novel dioscorin isoforms from Dioscorea japonica. Plant Sci. 2012 Feb;183:14-9. PMID:22195572 doi:10.1016/j.plantsci.2011.10.021

[2] Jheng YJ, Tsai WY, Chen KH, Lin KW, Chyan CL, Yang CC, Lin KC. Recombinant dioscorins of the yam storage protein expressed in Escherichia coli exhibit antioxidant and immunomodulatory activities. Protein Expr Purif. 2012 Sep;85(1):77-85. PMID:22796748 doi:10.1016/j.pep.2012.07.001

[3] Xue YL, Miyakawa T, Nakamura A, Hatano K, Sawano Y, Tanokura M. Yam Tuber Storage Protein Reduces Plant Oxidants Using the Coupled Reactions as Carbonic Anhydrase and Dehydroascorbate Reductase. Mol Plant. 2015 Jul;8(7):1115-8. PMID:25747844 doi:10.1016/j.molp.2015.02.015

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4twl

Crystal structure of dioscorin complexed with ascorbate

Structural highlights

Function

References

Contents

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