6cjd

Publication Abstract from PubMed

Type III protein secretion systems (T3SS) are encoded by several pathogenic or symbiotic bacteria. The central component of this nanomachine is the needle complex. Here we show in a Salmonella Typhimurium T3SS that assembly of the needle filament of this structure requires OrgC, a protein encoded within the T3SS gene cluster. Absence of OrgC results in significantly reduced number of needle substructures but does not affect needle length. We show that OrgC is secreted by the T3SS and that exogenous addition of OrgC can complement a orgC mutation. We also show that OrgC interacts with the needle filament subunit PrgI and accelerates its polymerization into filaments in vitro. The structure of OrgC shows a novel fold with a shared topology with a domain from flagellar capping proteins. These findings identify a novel component of T3SS and provide new insight into the assembly of the type III secretion machine.

A protein secreted by the Salmonella type III secretion system controls needle filament assembly.,Kato J, Dey S, Soto JE, Butan C, Wilkinson MC, De Guzman RN, Galan JE Elife. 2018 Jul 17;7. pii: 35886. doi: 10.7554/eLife.35886. PMID:30015613^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.