6e1j

Crystal Structure of Methylthioalkylmalate Synthase (BjuMAM1.1) from Brassica juncea

spinqualitylabelsall models 6e1j, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6e1j is a 2 chain structure with sequence from Brassica juncea. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.096Å Ligands: COA, KMT, MN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function C5J4P1_BRAJU Publication Abstract from PubMed Methylthioalkylmalate synthase catalyzes the committed step in the side-chain elongation of methionine-derived aliphatic glucosinolates and likely evolved from the isopropylmalate synthases of leucine biosynthesis. The globally cultivated Brassica species possess diverse aliphatic glucosinolates important for plant defense and animal nutrition; however, the molecular basis for the evolution of methylthioalkylmalate synthase and its generation of natural product diversity in Brassica is poorly understood. Here we show that Brassica genomes encode multiple methylthioalkylmalate synthase that have differences in expression profiles and 2-oxo substrate preference that account for diversity of aliphatic glucosinolates across Brassica accessions. The 2.1 A resolution x-ray crystal structure of B. juncea methylthioalkylmalate synthase identifies key active site residues responsible for controlling specificity for different 2-oxo substrates and the determinants of side-chain length in aliphatic glucosinolates. Overall, these results provide the evolutionary and biochemical foundation for diversification of glucosinolates profiles across globally-cultivated Brassica species, which could be used with ongoing breeding strategies towards manipulation of beneficial glucosinolates compounds for animal health and plant protection. Molecular Basis of the Evolution of Methylthioalkylmalate Synthase and Diversity of Methionine-Derived Glucosinolates.,Kumar R, Lee SG, Augustine R, Reichelt M, Vassao DG, Palavalli MH, Allen A, Gershenzon J, Jez JM, Bisht NC Plant Cell. 2019 Apr 25. pii: tpc.19.00046. doi: 10.1105/tpc.19.00046. PMID:31023839 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also 2-isopropylmalate synthase 3D structures

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