6fdb

Positively supercharged variant of the computationally designed cage protein O3-33

spinqualitylabelsall models 6fdb, resolution 3.62Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6fdb is a 8 chain structure with sequence from "bacillus_cholerae-suis"_smith_1894 "bacillus cholerae-suis" smith 1894. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: A7S24_10320, A7S72_11360, BUJ19_10850, CBI64_04620, CEP91_14370, IN36_13565, IN77_10790, IN95_23850, NGUA18_00608 ("Bacillus cholerae-suis" Smith 1894) Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Oligonucleotide therapeutics have transformative potential in modern medicine but are poor drug candidates in themselves unless fitted with compensatory carrier systems. We describe a simple approach to transform a designed porous protein cage into a nucleic acid delivery vehicle. By introducing arginine mutations to the lumenal surface, a positively supercharged capsule is created, which can encapsidate oligonucleotides in vitro with high binding affinity. We demonstrate that the siRNA-loaded cage is taken up by mammalian cells and releases its cargo to induce RNAi and knockdown gene expression. These general concepts could also be applied to alternative scaffold designs, expediting the development of artificial protein cages toward delivery applications. Rational Engineering of a Designed Protein Cage for siRNA Delivery.,Edwardson TGW, Mori T, Hilvert D J Am Chem Soc. 2018 Aug 9. doi: 10.1021/jacs.8b06442. PMID:30091604 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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