6gen

Chromatin remodeller-nucleosome complex at 4.5 A resolution.

Loading... Status: waiting for Mol* initialization Structural highlights 6gen is a 14 chain structure with sequence from Saccharomyces cerevisiae S288C and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.6Å Ligands: ADP, BEF, MG, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The yeast SWR1 complex exchanges histone H2A in nucleosomes with Htz1 (H2A.Z in humans). The cryo-electron microscopy structure of the SWR1 complex bound to a nucleosome at 3.6-angstrom resolution reveals details of the intricate interactions between components of the SWR1 complex and its nucleosome substrate. Interactions between the Swr1 motor domains and the DNA wrap at superhelical location 2 distort the DNA, causing a bulge with concomitant translocation of the DNA by one base pair, coupled to conformational changes of the histone core. Furthermore, partial unwrapping of the DNA from the histone core takes place upon binding of nucleosomes to SWR1 complex. The unwrapping, as monitored by single-molecule data, is stabilized and has its dynamics altered by adenosine triphosphate binding but does not require hydrolysis. Structure and dynamics of the yeast SWR1-nucleosome complex.,Willhoft O, Ghoneim M, Lin CL, Chua EYD, Wilkinson M, Chaban Y, Ayala R, McCormack EA, Ocloo L, Rueda DS, Wigley DB Science. 2018 Oct 12;362(6411). pii: 362/6411/eaat7716. doi:, 10.1126/science.aat7716. PMID:30309918 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Actin-related protein 3D structures Helicase 3D structures Histone 3D structures Vacuolar protein sorting-associated protein 3D structures

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