6q8u

Structure of the standard kink turn HmKt-7 variant A2bm6A bound with AfL7Ae protein

spinqualitylabelsall models 6q8u, resolution 1.99Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6q8u is a 4 chain structure with sequence from Archaeoglobus fulgidus DSM 4304 and Haloarcula marismortui. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.99Å Ligands: 6MZ, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function RL7A_ARCFU Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, box H/ACA and box C/D sRNAs. Publication Abstract from PubMed N(6)-methyladenine is the most common covalent modification in cellular RNA species, with demonstrated functional consequences. At the molecular level this methylation could alter local RNA structure, and/or modulate the binding of specific proteins. We have previously shown that trans-Hoogsteen-sugar (sheared) A:G base pairs can be completely disrupted by methylation, and that this occurs in a sub-set ofD/D k-turn structures. In this work we have investigated to what extent sequence context affects the severity with which inclusion of N(6)-methyladenine into different A:G base pairs of a standard k-turn affects RNA folding and L7Ae protein binding. We find that local sequence has a major influence, ranging from complete absence of folding and protein binding to a relatively mild effect. We have determined the crystal structure of one of these species both free and protein-bound, showing the environment of the methyl group and the way the modification is accommodated into the k-turn structure. Effect of methylation of adenine N(6) on kink turn structure depends on location.,Ashraf S, Huang L, Lilley DMJ RNA Biol. 2019 Jun 24:1-9. doi: 10.1080/15476286.2019.1630797. PMID:31234702 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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