6toy

Crystal structure of Bacillus paralicheniformis wild-type alpha-amylase

spinqualitylabelsall models 6toy, resolution 1.95Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6toy is a 1 chain structure with sequence from Bacillus licheniformis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.95Å Ligands: ACY, CA, FMT, MLI, NA Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function I3P686_BACLI Publication Abstract from PubMed alpha-Amylase from Bacillus paralicheniformis (BliAmy), belonging to GH13_5 subfamily of glycoside hydrolases, was proven to be a highly efficient raw starch digesting enzyme. The ability of some alpha-amylases to hydrolyze raw starch is related to the existence of surface binding sites (SBSs) for polysaccharides that can be distant from the active site. Crystallographic studies performed on BliAmy in the apo form and of enzyme bound with different oligosaccharides and oligosaccharide precursors revealed binding of these ligands to one SBS with two amino acids F257 and Y358 mainly involved in complex formation. The role of this SBS in starch binding and degradation was probed by designing enzyme variants mutated in this region (F257A and Y358A). Kinetic studies with different substrates show that starch binding through the SBS is disrupted in the mutants and that F257 and Y358 contributed cumulatively to binding and hydrolysis. Mutation of both sites (F257A/Y358A) resulted in a 5-fold lower efficacy with raw starch as substrate and at least 5.5-fold weaker binding compared to the wild type BliAmy, suggesting that the ability of BliAmy to hydrolyze raw starch with high efficiency is related to the level of its adsorption onto starch granules. Characterization of the starch surface binding site on Bacillus paralicheniformis alpha-amylase.,Bozic N, Rozeboom HJ, Loncar N, Slavic MS, Janssen DB, Vujcic Z Int J Biol Macromol. 2020 Oct 12. pii: S0141-8130(20)34647-X. doi:, 10.1016/j.ijbiomac.2020.10.025. PMID:33058974 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Amylase 3D structures

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