6w2v

Junction 23, DHR14-DHR18

spinqualitylabelsall models 6w2v, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.399Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed The ability to precisely design large proteins with diverse shapes would enable applications ranging from the design of protein binders that wrap around their target to the positioning of multiple functional sites in specified orientations. We describe a protein backbone design method for generating a wide range of rigid fusions between helix-containing proteins and use it to design 75,000 structurally unique junctions between monomeric and homo-oligomeric de novo designed and ankyrin repeat proteins (RPs). Of the junction designs that were experimentally characterized, 82% have circular dichroism and solution small-angle X-ray scattering profiles consistent with the design models and are stable at 95 degrees C. Crystal structures of four designed junctions were in close agreement with the design models with rmsds ranging from 0.9 to 1.6 A. Electron microscopic images of extended tetrameric structures and approximately 10-nm-diameter "L" and "V" shapes generated using the junctions are close to the design models, demonstrating the control the rigid junctions provide for protein shape sculpting over multiple nanometer length scales. Modular repeat protein sculpting using rigid helical junctions.,Brunette TJ, Bick MJ, Hansen JM, Chow CM, Kollman JM, Baker D Proc Natl Acad Sci U S A. 2020 Apr 3. pii: 1908768117. doi:, 10.1073/pnas.1908768117. PMID:32245816 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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