We apologize for Proteopedia being slow to respond. For the past two years, a new implementation of Proteopedia has been being built. Soon, it will replace this 18-year old system. All existing content will be moved to the new system at a date that will be announced here.

6yle

From Proteopedia

Jump to: navigation, search

Rix1-Rea1 pre-60S particle - Rix1-subcomplex, body 3 (rigid body refinement)

Structural highlights

6yle is a 5 chain structure with sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.3Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

IPI3_YEAST Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA.^[1] ^[2]

Publication Abstract from PubMed

Ribosome assembly is driven by numerous assembly factors, including the Rix1 complex and the AAA ATPase Rea1. These two assembly factors catalyze 60S maturation at two distinct states, triggering poorly understood large-scale structural transitions that we analyzed by cryo-electron microscopy. Two nuclear pre-60S intermediates were discovered that represent previously unknown states after Rea1-mediated removal of the Ytm1-Erb1 complex and reveal how the L1 stalk develops from a pre-mature nucleolar to a mature-like nucleoplasmic state. A later pre-60S intermediate shows how the central protuberance arises, assisted by the nearby Rix1-Rea1 machinery, which was solved in its pre-ribosomal context to molecular resolution. This revealed a Rix12-Ipi32 tetramer anchored to the pre-60S via Ipi1, strategically positioned to monitor this decisive remodeling. These results are consistent with a general underlying principle that temporarily stabilized immature RNA domains are successively remodeled by assembly factors, thereby ensuring failsafe assembly progression.

Construction of the Central Protuberance and L1 Stalk during 60S Subunit Biogenesis.,Kater L, Mitterer V, Thoms M, Cheng J, Berninghausen O, Beckmann R, Hurt E Mol Cell. 2020 Aug 20;79(4):615-628.e5. doi: 10.1016/j.molcel.2020.06.032. Epub, 2020 Jul 14. PMID:32668200^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Krogan NJ, Peng WT, Cagney G, Robinson MD, Haw R, Zhong G, Guo X, Zhang X, Canadien V, Richards DP, Beattie BK, Lalev A, Zhang W, Davierwala AP, Mnaimneh S, Starostine A, Tikuisis AP, Grigull J, Datta N, Bray JE, Hughes TR, Emili A, Greenblatt JF. High-definition macromolecular composition of yeast RNA-processing complexes. Mol Cell. 2004 Jan 30;13(2):225-39. PMID:14759368 doi:10.1016/s1097-2765(04)00003-6
↑ Galani K, Nissan TA, Petfalski E, Tollervey D, Hurt E. Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits. J Biol Chem. 2004 Dec 31;279(53):55411-8. Epub 2004 Nov 3. PMID:15528184 doi:http://dx.doi.org/10.1074/jbc.M406876200
↑ Kater L, Mitterer V, Thoms M, Cheng J, Berninghausen O, Beckmann R, Hurt E. Construction of the Central Protuberance and L1 Stalk during 60S Subunit Biogenesis. Mol Cell. 2020 Aug 20;79(4):615-628.e5. PMID:32668200 doi:10.1016/j.molcel.2020.06.032