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7wkc

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A prototype protein nanocage minimized from carboxysomes with gated oxygen permeability

Structural highlights

7wkc is a 5 chain structure with sequence from Thermosynechococcus vestitus BP-1. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CCML_THEVB Probably forms vertices in the carboxysome, a polyhedral inclusion where RuBisCO (ribulose bisphosphate carboxylase, rbcL-rbcS) is sequestered. Has been modeled to induce curvature upon insertion into an otherwise flat hexagonal molecular layer of CcmK subunits.[UniProtKB:P72759][HAMAP-Rule:MF_00858]

Publication Abstract from PubMed

Protein nanocages (PNCs) in cells and viruses have inspired the development of self-assembling protein nanomaterials for various purposes. Despite the successful creation of artificial PNCs, the de novo design of PNCs with defined permeability remains challenging. Here, we report a prototype oxygen-impermeable PNC (OIPNC) assembled from the vertex protein of the beta-carboxysome shell, CcmL, with quantum dots as the template via interfacial engineering. The structure of the cage was solved at the atomic scale by combined solid-state NMR spectroscopy and cryoelectron microscopy, showing icosahedral assembly of CcmL pentamers with highly conserved interpentamer interfaces. Moreover, a gating mechanism was established by reversibly blocking the pores of the cage with molecular patches. Thus, the oxygen permeability, which was probed by an oxygen sensor inside the cage, can be completely controlled. The CcmL OIPNC represents a PNC platform for oxygen-sensitive or oxygen-responsive storage, catalysis, delivery, sensing, etc.

A prototype protein nanocage minimized from carboxysomes with gated oxygen permeability.,Gao R, Tan H, Li S, Ma S, Tang Y, Zhang K, Zhang Z, Fan Q, Yang J, Zhang XE, Li F Proc Natl Acad Sci U S A. 2022 Feb 1;119(5). pii: 2104964119. doi:, 10.1073/pnas.2104964119. PMID:35078933^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gao R, Tan H, Li S, Ma S, Tang Y, Zhang K, Zhang Z, Fan Q, Yang J, Zhang XE, Li F. A prototype protein nanocage minimized from carboxysomes with gated oxygen permeability. Proc Natl Acad Sci U S A. 2022 Feb 1;119(5):e2104964119. PMID:35078933 doi:10.1073/pnas.2104964119