8bz7

Crystal structure of the L. monocytogenes RmlT in complex with TDP-rhamnose

spinqualitylabelsall models 8bz7, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8bz7 is a 6 chain structure with sequence from Listeria monocytogenes. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.2Å Ligands: CL, GOL, MG, TAR, TRH Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A401AAP7_LISMN Publication Abstract from PubMed Wall teichoic acids (WTAs) from the major Gram-positive foodborne pathogen Listeria monocytogenes are peptidoglycan-associated glycopolymers decorated by monosaccharides that, while not essential for bacterial growth, are required for bacterial virulence and resistance to antimicrobials. Here we report the structure and function of a bacterial WTAs rhamnosyltransferase, RmlT, strictly required for L. monocytogenes WTAs rhamnosylation. In particular, we demonstrated that RmlT transfers rhamnose from dTDP-L-rhamnose to naked WTAs, and that specificity towards TDP-rhamnose is not determined by its binding affinity. Structures of RmlT with and without its substrates showed that this enzyme is a dimer, revealed the residues responsible for interaction with the substrates and that the catalytic residue pre-orients the acceptor substrate towards the nucleophilic attack to the sugar. Additionally, the structures provided indications for two potential interaction pathways for the long WTAs on the surface of RmlT. Finally, we confirmed that WTAs glycosyltransferases are promising targets for next-generation strategies against Gram-positive pathogens by showing that inactivation of the RmlT catalytic activity results in a decreased infection in vivo. Molecular properties of the RmlT wall teichoic acid rhamnosyltransferase that modulates virulence in Listeria monocytogenes.,Monteiro R, Cereija TB, Pombinho R, Voskuilen T, Codee JDC, Sousa S, Morais-Cabral JH, Cabanes D Nat Commun. 2025 Jan 2;16(1):24. doi: 10.1038/s41467-024-55360-1. PMID:39746981 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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