8i2q | pdb_00008i2q

Beijerinckia indica beta-fructosyltransferase variant H395R/F473Y

spinqualitylabelsall models 8i2q, resolution 2.15Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8i2q is a 1 chain structure with sequence from Beijerinckia indica subsp. indica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.15Å Ligands: GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B2IF78_BEII9 Publication Abstract from PubMed The trisaccharide 1-kestose, a major constituent of fructooligosaccharide, has strong prebiotic effects. We used high-performance liquid chromatography and 1H nuclear magnetic resonance spectroscopy to show that BiBftA, a beta-fructosyltransferase belonging to glycoside hydrolase family 68, from Beijerinckia indica subsp. indica catalyzes transfructosylation of sucrose to produce mostly 1-kestose and levan polysaccharides. We substituted His395 and Phe473 in BiBftA with Arg and Tyr, respectively, and analyzed the reactions of the mutant enzymes with 180 g/L sucrose. The ratio of the molar concentrations of glucose and 1-kestose in the reaction mixture with wild-type BiBftA was 100:8.1, whereas that in the reaction mixture with the variant H395R/F473Y was 100:45.5, indicating that H395R/F473Y predominantly accumulated 1-kestose from sucrose. The X-ray crystal structure of H395R/F473Y suggests that its catalytic pocket is unfavorable for binding of sucrose while favorable for transfructosylation. Characterization and alteration of product specificity of Beijerinckia indica subsp. indica beta-fructosyltransferase.,Li D, Miyasaka Y, Kubota A, Kozono T, Kitano Y, Sasaki N, Fujii T, Tochio T, Kadota Y, Nishikawa A, Tonozuka T Biosci Biotechnol Biochem. 2023 Aug 23;87(9):981-990. doi: 10.1093/bbb/zbad074. PMID:37280168 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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