8ijs

anti-VEGF nanobody mutant

spinqualitylabelsall models 8ijs, resolution 1.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8ijs is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.752Å Ligands: ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Wet age-related macular degeneration (wet AMD) is the most common cause of blindness, and chronic intravitreal injection of anti-vascular endothelial growth factor (VEGF) proteins has been the dominant therapeutic approach. Less intravitreal injection and a prolonged inter-injection interval are the main drivers behind new wet AMD drug innovations. By rationally engineering the surface residues of a model anti-VEGF nanobody, we obtained a series of anti-VEGF nanobodies with identical protein structures and VEGF binding affinities, while drastically different crystallization propensities and crystal lattice structures. Among these nanobody crystals, the P2(1)2(1)2(1) lattice appeared to be denser and released protein slower than the P1 lattice, while nanobody crystals embedding zinc coordination further slowed the protein release rate. The polymorphic protein crystals could be a potentially breakthrough strategy for chronic intravitreal administration of anti-VEGF proteins. Polymorphic nanobody crystals as long-acting intravitreal therapy for wet age-related macular degeneration.,Zhu S, Fan S, Tang T, Huang J, Zhou H, Huang C, Chen Y, Qian F Bioeng Transl Med. 2023 May 4;8(6):e10523. doi: 10.1002/btm2.10523. eCollection , 2023 Nov. PMID:38023710 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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