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8xcg

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Tail tip complex of bacteriophage lambda in the open state

Structural highlights

8xcg is a 15 chain structure with sequence from Escherichia phage Lambda. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Electron Microscopy, Resolution 3.46Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TIPM_LAMBD Part of the distal tail tip which plays a role in DNA ejection during entry, and in tail assembly initiation during exit. May bind tail tip complex associated with tape measure protein and allow tail tube protein polymerization on top of tail tip.^[1]

Publication Abstract from PubMed

Bacteriophage infection, a pivotal process in microbiology, initiates with the phage's tail recognizing and binding to the bacterial cell surface, which then mediates the injection of viral DNA. Although comprehensive studies on the interaction between bacteriophage lambda and its outer membrane receptor, LamB, have provided rich information about the system's biochemical properties, the precise molecular mechanism remains undetermined. This study revealed the high-resolution cryo-electron microscopy (cryo-EM) structures of the bacteriophage lambda tail complexed with its irreversible Shigella sonnei 3070 LamB receptor and the closed central tail fiber. These structures reveal the complex processes that trigger infection and demonstrate a substantial conformational change in the phage lambda tail tip upon LamB binding. Providing detailed structures of bacteriophage lambda infection initiation, this study contributes to the expanding knowledge of lambda-bacterial interaction, which holds significance in the fields of microbiology and therapeutic development.

Structural mechanism of bacteriophage lambda tail's interaction with the bacterial receptor.,Ge X, Wang J Nat Commun. 2024 May 17;15(1):4185. doi: 10.1038/s41467-024-48686-3. PMID:38760367^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Katsura I. Morphogenesis of bacteriophage lambda tail. Polymorphism in the assembly of the major tail protein. J Mol Biol. 1976 Nov 5;107(3):307-26. PMID:1003470 doi:10.1016/s0022-2836(76)80007-1
↑ Ge X, Wang J. Structural mechanism of bacteriophage lambda tail's interaction with the bacterial receptor. Nat Commun. 2024 May 17;15(1):4185. PMID:38760367 doi:10.1038/s41467-024-48686-3