9plw

The structure of O-glycopeptidase BcM60K from Bacteroides caccae in complex with a core 2 glycan

spinqualitylabelsall models 9plw, resolution 2.19Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9plw is a 2 chain structure with sequence from Bacteroides caccae ATCC 43185 and Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.19Å Ligands: A2G, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0AAW7WJM4_9BACE Publication Abstract from PubMed O-glycopeptidases are enzymes that hydrolyze the peptide bonds in glycoproteins by a mechanism that involves specific recognition of O-linked glycans on the substrate. Bacteroides caccae, an accomplished mucin degrader, is a member of the human gut microbiota with sixteen genes encoding putative O-glycopeptidases in the peptidase_M60 family. At present, the diversity of substrate selectivity in O-glycopeptidases is not well-understood nor is the rationale behind their expansion in bacteria such as B. caccae. Here we reveal the activity and diversity of the peptidase_M60 O-glycopeptidases encoded in the B. caccae genome. At least thirteen of the sixteen peptidase_M60 genes encode active mucinolytic enzymes. Targeted functional studies by a high-throughput FRET screen combined with detailed kinetic analyses reveal that five examples in an uncharacterized clade of peptidase_M60 proteins are specifically O-glycopeptidases with different substrate selectivities despite their relatively high degree of relatedness. Structural analyses of these enzymes, including bound complexes, reveal new insight into the molecular underpinnings of O-glycopeptidase diversity. This highlights the larger context of how varied the selectivity of peptidase_M60 O-glycopeptidases can be for the glycan moiety and/or the peptide portion of the substrates, and why mucin degraders like B. caccae diversify O-glycopeptidase substrate repertoires to potentially maximize breakdown of this extraordinarily complex polymer. Substrate recognition and cleavage by mucin degrading O-glycopeptidases from the gut microbe Bacteroides caccae.,Pluvinage B, Bourdon K, Canil O, Deventer A, Alvarez B, Mihalynuk L, Thompson N, Wakarchuk W, Boraston AB J Biol Chem. 2026 Jun 4:113222. doi: 10.1016/j.jbc.2026.113222. PMID:42248462 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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