9ye2 | pdb_00009ye2

Crystal Structure of the GRAM-4 antibody fragment in complex with NAGG peptide

spinqualitylabelsall models 9ye2, resolution 2.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9ye2 is a 3 chain structure with sequence from Homo sapiens and Plasmodium vivax. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.65Å Ligands: GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Circumsporozoite protein (CSP) is the most abundant Plasmodium sporozoite surface antigen. It is the major component of licensed Plasmodium falciparum vaccines and, hence, is the logical lead candidate for Plasmodium vivax (P. vivax) vaccines. However, our limited knowledge of protective B cell epitopes on P. vivax CSP remains a major roadblock. Here we identified two Plasmodium vivax CSP (PvCSP) B cell epitopes, PvCSP-A and PvCSP-B/NAGG, by screening inhibitory plasma from P. vivax-infected individuals. The epitopes were located in the N- and C-terminal regions of PvCSP, respectively, and were distinct from the canonical central repeat region epitope. We isolated a monoclonal antibody, GRAM-4, that recognized the PvCSP-B/NAGG epitope and mediated potent inhibition of traversal, hepatocyte invasion, and liver-stage development in vitro, confirming PvCSP-B/NAGG as a genuine neutralization target. Structural resolution of the GRAM-4:PvCSP-B/NAGG interaction at 2.65 A revealed the molecular basis of epitope recognition, providing a blueprint for PvCSP vaccine development. Targeting a site of vulnerability on circumsporozoite protein inhibits Plasmodium vivax malaria infection.,Visweswaran GRR, Mahdavi P, Vijayan K, Ntumngia FB, Staker BL, Barnes SJ, Subramani PA, Raappana A, Dambrauskas N, Glennon EKK, Kolli SK, Ogbondah MM, Watson A, Camargo N, Sankaran B, Thawornpan P, Jenwithisuk R, Myler PJ, Kappe SHI, Roobsoong W, Chootong P, Sattabongkot J, Kaushansky A, Vigdorovich V, Adams JH, Sather DN Immunity. 2026 Sep 24:S1074-7613(26)00376-6. doi: 10.1016/j.immuni.2026.09.001. PMID:42785294 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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