1cld

From Proteopedia

DNA-binding protein

Structural highlights

1cld is a 1 chain structure with sequence from Kluyveromyces lactis. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LAC9_KLULA Positive regulatory protein, that controls induction of the lactose-galactose regulation of Kluyveromyces lactis.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The Zn2Cys6 DNA-binding domain has been identified by sequence homology in approximately forty fungal proteins, including the K. lactis LAC9 transcriptional activator. Using 1H NMR spectroscopy, we have determined the solution structure of a cadmium-substituted form of the LAC9 DNA-binding domain. We have complemented this approach by applying a series of 113Cd-1H NMR experiments, including several novel heteroTOCSY-based techniques. The DNA-binding domain forms a core of two alpha-helix/extended strand segments around the Cd2 binuclear cluster, with a network of amide proton-cysteinyl S gamma hydrogen bonds stabilizing the cluster. Comparison with other Zn2Cys6 domain structures provides insight into the common structural elements used in metal coordination and DNA binding.

Solution structure of the Kluyveromyces lactis LAC9 Cd2 Cys6 DNA-binding domain.,Gardner KH, Anderson SF, Coleman JE Nat Struct Biol. 1995 Oct;2(10):898-905. PMID:7552715^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gardner KH, Anderson SF, Coleman JE. Solution structure of the Kluyveromyces lactis LAC9 Cd2 Cys6 DNA-binding domain. Nat Struct Biol. 1995 Oct;2(10):898-905. PMID:7552715