1gmg

From Proteopedia

ALANINE 31 PROLINE MUTANT OF ROP PROTEIN, MONOCLINIC FORM

Structural highlights

1gmg is a 2 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.9Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ROP_ECOLX Regulates plasmid DNA replication by modulating the initiation of transcription of the primer RNA precursor. Processing of the precursor of the primer, RNAII, is inhibited by hydrogen bonding of RNAII to its complementary sequence in RNAI. ROP increases the affinity of RNAI for RNAII and thus decreases the rate of replication initiation events.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structure of a 4-alpha-helical bundle protein has been determined by the application of a 23-dimensional molecular-replacement search performed using a stochastic method. The search model for the calculation was a 26-residue-long polyalanine helix amounting to less than 13% of the total number of atoms in the asymmetric unit of the target crystal structure. The crystal structure determination procedure is presented in detail, with emphasis on the molecular-replacement calculations.

Structure determination of a small protein through a 23-dimensional molecular-replacement search.,Glykos NM, Kokkinidis M Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):709-18. Epub 2003, Mar 25. PMID:12657790^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Glykos NM, Kokkinidis M. Structure determination of a small protein through a 23-dimensional molecular-replacement search. Acta Crystallogr D Biol Crystallogr. 2003 Apr;59(Pt 4):709-18. Epub 2003, Mar 25. PMID:12657790