1ri0 | pdb_00001ri0
NMR structure of the N-terminal hath domain of human HDGF
Structural highlights
FunctionHDGF_HUMAN Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.[1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedHepatoma-derived growth factor (HDGF)-related proteins (HRPs) comprise a new protein family that has been implicated in nephrogenesis, tumorigenesis, vascular development, cell proliferation, and transcriptional activation. All HRPs share a conserved N-terminal homologous to the amino terminus of HDGF (HATH) domain, but vary significantly in the C-terminal region. Here, we show that in solution the N and C termini of human HDGF form two structurally independent domains. The 100 amino acid residue N-terminal HATH domain is well-structured while the 140 amino acid residue C-terminal domain is disordered. We determined the solution structure of the HATH domain by NMR. The core structure of the HATH domain is a five-stranded beta-barrel followed by two alpha-helices, similar to those of PWWP domains of known structures. Surface plasmon resonance results showed that the HATH domain is primarily responsible for heparin binding. On the basis of the chemical shift perturbation induced by binding of heparin-derived hexasaccharide, we identified a prominent, highly positively charged region as the putative heparin-binding site. Sequence comparison and structure prediction suggest that all HRPs are likely to adapt a similar modular structure. Solution structure and heparin interaction of human hepatoma-derived growth factor.,Sue SC, Chen JY, Lee SC, Wu WG, Huang TH J Mol Biol. 2004 Nov 5;343(5):1365-77. PMID:15491618[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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