1yx9

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Effect of Dimethyl Sulphoxide on the crystal structure of Porcine Pepsin

Structural highlights

1yx9 is a 1 chain structure with sequence from Sus scrofa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3Å
Ligands:DMS
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PEPA_PIG Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The structure of porcine pepsin crystallized in the presence of dimethyl sulphoxide has been analysed by X-ray crystallography to obtain insights into the structural events that occur at the onset of chemical denaturation of proteins. The results show that one dimethyl sulphoxide molecule occupies a site on the surface of pepsin interacting with two of its residues. An increase in the average temperature factor of pepsin in the presence of dimethyl sulphoxide has been observed indicating protein destabilization induced by the denaturant. Significant increase in the temperature factor and weakening of the electron density have been observed for the catalytic water molecule located between the active aspartates. The conformation of pepsin remains unchanged in the crystal structure. However, the enzyme assay and circular dichroism studies indicate that dimethyl sulphoxide causes a slight change in the secondary structure and complete loss of activity of pepsin in solution.

Effect of dimethyl sulphoxide on the crystal structure of porcine pepsin.,Kesavulu MM, Ramasubramanian S, Suguna K Biochem Biophys Res Commun. 2005 Jun 17;331(4):1510-4. PMID:15883044[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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See Also

References

  1. ↑ Kesavulu MM, Ramasubramanian S, Suguna K. Effect of dimethyl sulphoxide on the crystal structure of porcine pepsin. Biochem Biophys Res Commun. 2005 Jun 17;331(4):1510-4. PMID:15883044 doi:10.1016/j.bbrc.2005.03.247

Contents


PDB ID 1yx9

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