2bgw

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XPF from Aeropyrum pernix, complex with DNA

Structural highlights

2bgw is a 4 chain structure with sequence from Aeropyrum pernix and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.8Å
Ligands:MG, SO4
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9YC15_AERPE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The XPF/Mus81 structure-specific endonucleases cleave double-stranded DNA (dsDNA) within asymmetric branched DNA substrates and play an essential role in nucleotide excision repair, recombination and genome integrity. We report the structure of an archaeal XPF homodimer alone and bound to dsDNA. Superposition of these structures reveals a large domain movement upon binding DNA, indicating how the (HhH)(2) domain and the nuclease domain are coupled to allow the recognition of double-stranded/single-stranded DNA junctions. We identify two nonequivalent DNA-binding sites and propose a model in which XPF distorts the 3' flap substrate in order to engage both binding sites and promote strand cleavage. The model rationalises published biochemical data and implies a novel role for the ERCC1 subunit of eukaryotic XPF complexes.

Structure of an XPF endonuclease with and without DNA suggests a model for substrate recognition.,Newman M, Murray-Rust J, Lally J, Rudolf J, Fadden A, Knowles PP, White MF, McDonald NQ EMBO J. 2005 Mar 9;24(5):895-905. Epub 2005 Feb 17. PMID:15719018[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
18 reviews cite this structure
Making and breaking nucleic acids: two-Mg2+-ion catalysis and substrate specificity.
Yang et al. (2006)
17 more
50 other articles
No citations found

See Also

References

  1. ↑ Newman M, Murray-Rust J, Lally J, Rudolf J, Fadden A, Knowles PP, White MF, McDonald NQ. Structure of an XPF endonuclease with and without DNA suggests a model for substrate recognition. EMBO J. 2005 Mar 9;24(5):895-905. Epub 2005 Feb 17. PMID:15719018

Contents


PDB ID 2bgw

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OCA

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