2cio

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The high resolution x-ray structure of papain complexed with fragments of the Trypanosoma brucei cysteine protease inhibitor ICP.

Structural highlights

2cio is a 2 chain structure with sequence from Carica papaya and Trypanosoma brucei. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.5Å
Ligands:ACT, GOL, OCS
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q868H0_9TRYP

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Attempts to cocrystallize the cysteine protease papain derived from the latex of Carica papaya with an inhibitor of cysteine proteases (ICP) from Trypanosoma brucei were unsuccessful. However, crystals of papain that diffracted to higher resolution, 1.5 A, than other crystals of this archetypal cysteine protease were obtained, so the analysis was continued. Surprisingly, the substrate-binding cleft was occupied by two short peptide fragments which have been assigned as remnants of ICP. Comparisons reveal that these peptides bind in the active site in a manner similar to that of the human cysteine protease inhibitor stefin B when it is complexed to papain. The assignment of the fragment sequences is consistent with the specificity of the protease.

High-resolution complex of papain with remnants of a cysteine protease inhibitor derived from Trypanosoma brucei.,Alphey MS, Hunter WN Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jun 1;62(Pt, 6):504-8. Epub 2006 May 5. PMID:16754967[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
0 reviews cite this structure
Cathepsin-L can resist lysis by human serum in Trypanosoma brucei brucei.
Alsford et al. (2014)
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2 other articles
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See Also

References

  1. ↑ Alphey MS, Hunter WN. High-resolution complex of papain with remnants of a cysteine protease inhibitor derived from Trypanosoma brucei. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jun 1;62(Pt, 6):504-8. Epub 2006 May 5. PMID:16754967 doi:10.1107/S1744309106014849

Contents


PDB ID 2cio

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OCA

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