Crystal Structure of Bacillus stearothermophilus PrfA-Holliday Junction Resolvase
Structural highlights
2fco is a 2 chain structure with sequence from Geobacillus kaustophilus HTA426. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
RECU_GEOKA Endonuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves mobile four-strand junctions by introducing symmetrical nicks in paired strands. Promotes annealing of linear ssDNA with homologous dsDNA. Required for DNA repair, homologous recombination and chromosome segregation.[HAMAP-Rule:MF_00130][1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
↑ Rigden DJ, Setlow P, Setlow B, Bagyan I, Stein RA, Jedrzejas MJ. PrfA protein of Bacillus species: prediction and demonstration of endonuclease activity on DNA. Protein Sci. 2002 Oct;11(10):2370-81. PMID:12237459 doi:10.1110/ps.0216802
