2jp3
From Proteopedia
Solution Structure of the human FXYD4 (CHIF) protein in SDS micelles
Structural highlights
FunctionFXYD4_RAT Induces a potassium channel when expressed in Xenopus oocytes. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe anisotropic spin interactions measured for membrane proteins in weakly oriented micelles and in oriented lipid bilayers provide independent and potentially complementary high-resolution restraints for structure determination. Here we show that the membrane protein CHIF adopts a similar structure in lipid micelles and bilayers, allowing the restraints from micelle and bilayer samples to be combined in a complementary fashion to enhance the structural information. Back-calculation and assignment of the NMR spectrum of CHIF in oriented lipid bilayers, from the structure determined in micelles, provides additional restraints for structure determination as well as the global orientation of the protein in the membrane. The combined use of solution and solid-state NMR restraints also affords cross-validation for the structural analysis. Structural similarity of a membrane protein in micelles and membranes.,Franzin CM, Teriete P, Marassi FM J Am Chem Soc. 2007 Jul 4;129(26):8078-9. doi: 10.1021/ja0728371. Epub 2007 Jun , 13. PMID:17567018[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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