2mb0

Structural highlights

Function

RBMX_HUMAN RNA-binding protein that plays several role in the regulation of pre- and post-transcriptional processes. Implicated in tissue-specific regulation of gene transcription and alternative splicing of several pre-mRNAs. Binds to and stimulates transcription from the tumor suppressor TXNIP gene promoter; may thus be involved in tumor suppression. When associated with SAFB, binds to and stimulates transcription from the SREBF1 promoter. Associates with nascent mRNAs transcribed by RNA polymerase II. Component of the supraspliceosome complex that regulates pre-mRNA alternative splice site selection. Can either activate or suppress exon inclusion; acts additively with TRA2B to promote exon 7 inclusion of the survival motor neuron SMN2. Represses the splicing of MAPT/Tau exon 10. Binds preferentially to single-stranded 5'-CC[A/C]-rich RNA sequence motifs localized in a single-stranded conformation; probably binds RNA as a homodimer. Binds non-specifically to pre-mRNAs. Plays also a role in the cytoplasmic TNFR1 trafficking pathways; promotes both the IL-1-beta-mediated inducible proteolytic cleavage of TNFR1 ectodomains and the release of TNFR1 exosome-like vesicles to the extracellular compartment.^{[1] [2] [3] [4] [5] [6] [7]}

References

1. ↑ Hofmann Y, Wirth B. hnRNP-G promotes exon 7 inclusion of survival motor neuron (SMN) via direct interaction with Htra2-beta1. Hum Mol Genet. 2002 Aug 15;11(17):2037-49. PMID:12165565
2. ↑ Nasim MT, Chernova TK, Chowdhury HM, Yue BG, Eperon IC. HnRNP G and Tra2beta: opposite effects on splicing matched by antagonism in RNA binding. Hum Mol Genet. 2003 Jun 1;12(11):1337-48. PMID:12761049
3. ↑ Shin KH, Kang MK, Kim RH, Christensen R, Park NH. Heterogeneous nuclear ribonucleoprotein G shows tumor suppressive effect against oral squamous cell carcinoma cells. Clin Cancer Res. 2006 May 15;12(10):3222-8. PMID:16707624 doi:http://dx.doi.org/10.1158/1078-0432.CCR-05-2656
4. ↑ Adamik B, Islam A, Rouhani FN, Hawari FI, Zhang J, Levine SJ. An association between RBMX, a heterogeneous nuclear ribonucleoprotein, and ARTS-1 regulates extracellular TNFR1 release. Biochem Biophys Res Commun. 2008 Jul 4;371(3):505-9. doi:, 10.1016/j.bbrc.2008.04.103. Epub 2008 Apr 28. PMID:18445477 doi:http://dx.doi.org/10.1016/j.bbrc.2008.04.103
5. ↑ Shin KH, Kim RH, Kim RH, Kang MK, Park NH. hnRNP G elicits tumor-suppressive activity in part by upregulating the expression of Txnip. Biochem Biophys Res Commun. 2008 Aug 8;372(4):880-5. doi:, 10.1016/j.bbrc.2008.05.175. Epub 2008 Jun 9. PMID:18541147 doi:10.1016/j.bbrc.2008.05.175
6. ↑ Heinrich B, Zhang Z, Raitskin O, Hiller M, Benderska N, Hartmann AM, Bracco L, Elliott D, Ben-Ari S, Soreq H, Sperling J, Sperling R, Stamm S. Heterogeneous nuclear ribonucleoprotein G regulates splice site selection by binding to CC(A/C)-rich regions in pre-mRNA. J Biol Chem. 2009 May 22;284(21):14303-15. doi: 10.1074/jbc.M901026200. Epub 2009, Mar 12. PMID:19282290 doi:http://dx.doi.org/10.1074/jbc.M901026200
7. ↑ Kanhoush R, Beenders B, Perrin C, Moreau J, Bellini M, Penrad-Mobayed M. Novel domains in the hnRNP G/RBMX protein with distinct roles in RNA binding and targeting nascent transcripts. Nucleus. 2010 Jan-Feb;1(1):109-22. doi: 10.4161/nucl.1.1.10857. PMID:21327109 doi:http://dx.doi.org/10.4161/nucl.1.1.10857