2o9l

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AMBER refined NMR Structure of the Sigma-54 RpoN Domain Bound to the-24 Promoter Element

Structural highlights

2o9l is a 3 chain structure with sequence from Aquifex aeolicus. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:Solution NMR
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O66858_AQUAE

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The sigma subunit of bacterial RNA polymerase (RNAP) regulates gene expression by directing RNAP to specific promoters. Unlike sigma(70)-type proteins, the alternative sigma factor, sigma(54), requires interaction with an ATPase to open DNA. We present the solution structure of the C-terminal domain of sigma(54) bound to the -24 promoter element, in which the conserved RpoN box motif inserts into the major groove of the DNA. This structure elucidates the basis for sequence specific recognition of the -24 element, orients sigma(54) on the promoter, and suggests how the C-terminal domain of sigma(54) interacts with RNAP.

Structural basis of DNA recognition by the alternative sigma-factor, sigma54.,Doucleff M, Pelton JG, Lee PS, Nixon BT, Wemmer DE J Mol Biol. 2007 Jun 15;369(4):1070-8. Epub 2007 Apr 12. PMID:17481658[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
10 reviews cite this structure
The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.
Bush et al. (2012)
9 more
21 other articles
No citations found

See Also

References

  1. ↑ Doucleff M, Pelton JG, Lee PS, Nixon BT, Wemmer DE. Structural basis of DNA recognition by the alternative sigma-factor, sigma54. J Mol Biol. 2007 Jun 15;369(4):1070-8. Epub 2007 Apr 12. PMID:17481658 doi:10.1016/j.jmb.2007.04.019

Contents


PDB ID 2o9l

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