3ahw

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Crystal Structure of Ustilago sphaerogena Ribonuclease U2 complexed with adenosine 2'-monophosphate

Structural highlights

3ahw is a 1 chain structure with sequence from Ustilago sphaerogena. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.03Å
Ligands:2AM, CA
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RNU2_USTSP

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Asparagine can be non-enzymatically deamidated and isomerized via succinimide to isoaspartate. This post-translational modification can potentially alter the physical properties or the function of the parent protein. Asn32 of ribonuclease U2A from Ustilago sphaerogena is known to rapidly deamidate and isomerize in alkaline conditions. The crystal structure of ribonuclease U2A complexed with 2'-adenylic acid and calcium ions was determined at 1.03 A resolution. In this structure, the region from Asp29 to Asp37 winds around a calcium ion, and the main-chain of Asn32-Gly33 adopts an extended conformation. Rotation of the side-chain of Asn32 could bring Asn32C(gamma) into close proximity to Gly33N, in a conformation suitable for succinimide formation. The structure suggests that in solution the region around Asn32-Gly33 is likely to be in equilibrium between multiple conformers, with the deamidation of Asn32 proceeding when the region adopts an extended conformation.

Conformational variation revealed by the crystal structure of RNase U2A complexed with Ca Ion and 2'-adenylic acid at 1.03 A resolution.,Noguchi S Protein Pept Lett. 2010;17(12):1559-61. PMID:20858208[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
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Protein asparagine deamidation prediction based on structures with machine learning methods.
Jia et al. (2017)
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3 other articles
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See Also

References

  1. ↑ Noguchi S. Conformational variation revealed by the crystal structure of RNase U2A complexed with Ca Ion and 2'-adenylic acid at 1.03 A resolution. Protein Pept Lett. 2010;17(12):1559-61. PMID:20858208

Contents


PDB ID 3ahw

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OCA

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