3bam

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RESTRICTION ENDONUCLEASE BAMHI COMPLEX WITH DNA AND MANGANESE IONS (POST-REACTIVE COMPLEX)

Structural highlights

3bam is a 5 chain structure with sequence from Bacillus amyloliquefaciens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.8Å
Ligands:MN
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

T2BA_BACAM Recognizes the double-stranded sequence GGATCC and cleaves after G-1.

Publication Abstract from PubMed

Type II restriction enzymes are characterized by their remarkable specificity and simplicity. They require only divalent metals (such as Mg2+ or Mn2+) as cofactors to catalyze the hydrolysis of DNA. However, most of the structural work on endonucleases has been performed in the absence of metals, leaving unanswered questions about their mechanisms of DNA cleavage. Here we report structures of the endonuclease BamHI-DNA complex, determined in the presence of Mn2+ and Ca2+, that describe the enzyme at different stages of catalysis. Overall, the results support a two-metal mechanism of DNA cleavage for BamHI which is distinct from that of EcoRV.

The role of metals in catalysis by the restriction endonuclease BamHI.,Viadiu H, Aggarwal AK Nat Struct Biol. 1998 Oct;5(10):910-6. PMID:9783752[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
15 reviews cite this structure
Making and breaking nucleic acids: two-Mg2+-ion catalysis and substrate specificity.
Yang et al. (2006)
14 more
59 other articles
No citations found

See Also

References

  1. ↑ Viadiu H, Aggarwal AK. The role of metals in catalysis by the restriction endonuclease BamHI. Nat Struct Biol. 1998 Oct;5(10):910-6. PMID:9783752 doi:10.1038/2352

Contents


PDB ID 3bam

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