Structural highlights
Function
EIZFM_STRCO Involved in the biosynthesis of the sesquiterpenoid antibiotic albaflavenone. Catalyzes the two-step allylic oxidation of epi-isozizaene to albaflavenone. First carries out a non-stereo-specific oxidation of epi-isozizaene to give a mixture of the albaflavenol epimers ((5R)-albaflavenol and (5S)-albaflavenol), each of which can serve as substrate for the second oxidation to yield albaflavenone. This is quite different from most other P450s which catalyze regio- and stereospecific oxidation. Displays also a farnesene synthase activity with farnesyl diphosphate (FPP) as substrate.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
- ↑ Zhao B, Lin X, Lei L, Lamb DC, Kelly SL, Waterman MR, Cane DE. Biosynthesis of the sesquiterpene antibiotic albaflavenone in Streptomyces coelicolor A3(2). J Biol Chem. 2008 Mar 28;283(13):8183-9. doi: 10.1074/jbc.M710421200. Epub 2008, Jan 30. PMID:18234666 doi:http://dx.doi.org/10.1074/jbc.M710421200
- ↑ Zhao B, Lei L, Vassylyev DG, Lin X, Cane DE, Kelly SL, Yuan H, Lamb DC, Waterman MR. Crystal structure of albaflavenone monooxygenase containing a moonlighting terpene synthase active site. J Biol Chem. 2009 Dec 25;284(52):36711-9. Epub 2009 Oct 26. PMID:19858213 doi:10.1074/jbc.M109.064683
