3edc

From Proteopedia

Jump to: navigation, search

Crystal Structure of a 1.6-hexanediol Bound Tetrameric Form of Escherichia coli Lac-repressor Refined to 2.1 Resolution

Structural highlights

3edc is a 4 chain structure with sequence from Escherichia coli K-12. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.1Å
Ligands:HEZ
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LACI_ECOLI Repressor of the lactose operon. Binds allolactose as an inducer.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We report the structure of a novel tetrameric form of the lactose repressor (LacI) protein from Escherichia coli refined to 2.1 A resolution. The tetramer is bound to 1.6-hexanediol present in the crystallization solution and the final R(free) for the structure is 0.201. The structure confirms previously reported structures on the monomer level. However, the tetramer is much more densely packed. This adds a new level of complexity to the interpretation of mutational effects and challenges details in the current model for LacI function. Several amino acids, previously associated with changes in function but unexplained at the structural level, appear in a new structural context in this tetramer which provides new implications for their function. Proteins 2009. (c) 2008 Wiley-Liss, Inc.

Crystal structure of a 1.6-hexanediol bound tetrameric form of Escherichia coli Lac-repressor refined to 2.1 A resolution.,Stenberg KA, Vihinen M Proteins. 2008 Sep 30. PMID:19004002[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Loading citation details..
Citations
reviews cite this structure
-1 more
other articles
No citations found

See Also

References

  1. ↑ Stenberg KA, Vihinen M. Crystal structure of a 1.6-hexanediol bound tetrameric form of Escherichia coli Lac-repressor refined to 2.1 A resolution. Proteins. 2008 Sep 30. PMID:19004002 doi:10.1002/prot.22284

Contents


PDB ID 3edc

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://proteopedia.org/wiki/index.php/3edc"
Personal tools