3gi9

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Crystal Structure of ApcT Transporter Bound to 7F11 Monoclonal Fab Fragment

Structural highlights

3gi9 is a 3 chain structure with sequence from Methanocaldococcus jannaschii and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.48Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Y609_METJA

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Amino acid, polyamine, and organocation (APC) transporters are secondary transporters that play essential roles in nutrient uptake, neurotransmitter recycling, ionic homeostasis, and regulation of cell volume. Here, we present the crystal structure of apo-ApcT, a proton-coupled broad-specificity amino acid transporter, at 2.35 A resolution. The structure contains 12 transmembrane helices, with the first 10 consisting of an inverted structural repeat of 5 transmembrane helices like LeuT. The ApcT structure reveals an inward-facing, apo state and an amine moiety of Lys158 located in a position equivalent to the Na2 ion of LeuT. We propose that Lys158 is central to proton-coupled transport and that the amine group serves the same functional role as the Na2 ion in LeuT, thus demonstrating common principles among proton- and sodium-coupled transporters.

Structure and Mechanism of a Na+ Independent Amino Acid Transporter.,Shaffer PL, Goehring A, Shankaranarayanan A, Gouaux E Science. 2009 Jul 22. PMID:19608859[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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Citations
45 reviews cite this structure
SLC6 neurotransmitter transporters: structure, function, and regulation.
Kristensen et al. (2011)
44 more
151 other articles
No citations found

See Also

References

  1. ↑ Shaffer PL, Goehring A, Shankaranarayanan A, Gouaux E. Structure and Mechanism of a Na+ Independent Amino Acid Transporter. Science. 2009 Jul 22. PMID:19608859

Contents


PDB ID 3gi9

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OCA

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